Table 2.
Small Bowel and Colon Histology and Immunohistochemistry (available in 28 patients)
| Data show mean ± SEM | GFD (n=14) | GCD (n=14) | P: effect of diet | |
|---|---|---|---|---|
| Duodenum | ||||
| IEL/100 epithelial cells | 28.5±5.0 | 23.5±6.1 | Ns | |
| CD3 lymphocytes/100 IEL | 30.5±5.4 | 23.9±5.5 | Ns | |
| CD4 lymphocytes# | ||||
| 1 | 1 | 4 | ||
| 2 | 11 | 10 | ||
| 3 | 1 | 0 | ||
| CD8 lymphocytes/100 IEL | 29.8±5.8 | 24.8±4.5 | Ns | |
| CD68 lymphocytes# | ||||
| 1 | 10 | 9 | ||
| 2 | 3 | 5 | ||
| CD79 lymphocytes# | ||||
| 1 | 1 | 3 | ||
| 2 | 11 | 10 | ||
| 3 | 1 | 1 | ||
| Mast cells #/hpf | 21.3±1.8 | 20.2±1.6 | Ns | |
| ZO-1 IHC | 152.3±22.3 | 165±21.5 | ||
| Villus:crypt ratio, % patients | ||||
| 3 | 38.5 | 35.7 | Ns | |
| 4 | 61.5 | 64.3 | Ns | |
| Colon | ||||
| IEL/100 epithelial cells | 3.1±0.4 | 2.6±0.5 | Ns | |
| CD4 lymphocytes# | ||||
| 1 | 13 | 9 | ||
| 2 | 0 | 5 | ||
| CD68 lymphocytes# | ||||
| 1 | 8 | 10 | ||
| 2 | 5 | 4 | ||
| CD79 lymphocytes# | ||||
| 1 | 9 | 10 | ||
| 2 | 3 | 3 | ||
| 3 | 1 | 1 | ||
| ZO-1 IHC | 115.4±19.8 | 135.7±18.6 | ||
#
CD4, CD68 and CD79a are scored semi-quantitatively (grade 1 to 4) and ZO-1immunohistochemistry was scored semi-quantitatively as described in methods section based on positive cells and intensity of staining. No statistical comparisons were conducted for the descriptive observations of CD4, CD68, CD79 lymphocytes and ZO-1 IHC, in contrast to the quantitative data on IELs, CD3, CD8 and mast cells.
Ns=non-significant