A, Western analysis of siRNA controls (siCon) and GRP78 knockdowns (siGRP78) transiently transfected as described and treated with and without cisplatin for 24, 48 and 72 hours. Ishikawa cells (upper) and AN3CA cells (lower) were probed for levels of GRP78, phospho-AKT, phospho-ERK, and total AKT. Vinculin was used as an internal loading control.