Fig. 2. Reduced emigration of 24p3−/− neutrophils.
(A) Total cellularity of the peritoneal exudates of casein challenged mice. Cellularity was enumerated in a hemocytometer. Error bars depict SD. (B) Flow cytometry analysis of neutrophils in the peritoneal exudates. Forward and Side scatter analysis. (C & D) Peritoneal exudates were stained with Gr-1 antibody and %positive cells were determined by flow cytometry. Values are average means of triplicate experiments with 2 mice per genotype per experiment. Error bars depict SD. (E) Flow cytometry analysis of bone marrow, peripheral blood and peritoneal exudates of casein challenged mice following staining with a Gr-1 PE antibody. Values are average means of triplicate experiments with 2 mice per genotype per experiment. Error bars depict SD. (F & G). CBA analysis of inflammatory cytokines in the sera of casein challenged mice. Values are average means of triplicate experiments with 2 mice per genotype per experiment. Error bars depict SD. (H & I). Quantitative determination of chemokines MIP-2 and KC in the sera of casein challenged mice. Values are average means of triplicate experiments with 2 mice per genotype per experiment. Error bars depict SD.
