Fig. 2.

The preparation of the MDI-HSA conjugates influences the 4,4′-MDI incorporation rates into HSA. The MDI-HSA preparations in volatile form show lower isocyanate incorporation rates when compared with conjugates prepared in-solution. MDI incorporation rate for various 4,4′-MDI conjugate prepared in-solution (i.s., filled square) and in-vapor (i.v., filled circle) was calculated as predicted number of MDI molecules per HSA molecule