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Agarose gel electrophoresis of nested-PCR products of P. jirovecii partial 18SrRNA gene. Lanes 1: positive control, lanes 9 and 13–15: positive clinical samples having specific 330 bp bands representative of P. jirovecii, lanes 2–8 and 10–12: no representative band indicating negative clinical samples, lane 16: negative control. Lanes 4 and 6 have unspecific bands with unexpected size seen in some samples. Lanes M: 100 bp DNA size marker.
