Figure 2.

HTS chemical screening in zebrafish identifies novel cyanide antidotes. A) Chemical structures of novel small-molecule antidotes identified in this screen. B) Survival curve of 6-dpf zebrafish treated with cyanide plus either novel antidotes identified in this screen or known antidotes. C) Effects of the combination of riboflavin plus a known cyanide antidote, hydroxocobalamin, on survival. D) Zebrafish embryos (30 hpf) were treated with a sublethal dose of cyanide and increasing doses of riboflavin for 1 h prior to the PMR assay. Each graph displays the mean motion index of 10 wells/condition over time. E) Excitation motion index for each condition. All data are represented as means ± se. *P ≤ 0.01.