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. 2013 Apr 23;8(4):e62247. doi: 10.1371/journal.pone.0062247

Figure 4. Determination of the Z-factor for 384-well and 1536-well assay.

Figure 4

(A) In a 384-well plate, 192 wells were used as a negative control (buffer only), represented by closed circles. An additional 192 wells were used as positive controls and were treated with CCG-50014, a potent RGS4 inhibitor, (10 µM final) represented by open circles [29]. The solid lines represent the mean value for the negative control and the positive control (1.74 and 0.92 respectively). The dashed lines marks the 3 standard deviation cut off for both the positive and negative control (standard deviation of 0.033 and 0.021 respectively). (B) This assay was conducted in 5.5% DMSO to mimic the actual concentration of DMSO in the pilot screen. In a 1536-well plate, 128 wells received buffer, negative control (closed symbols) and the remaining 128 wells received 10 µM final CCG-50014, positive control (open symbols). The solid lines represent the mean value for the negative control and the positive control (0.67 and 0.30 respectively). The dashed lines marks the 3 standard deviation cut off for both the positive and negative control (standard deviation of 0.028 and 0.021 respectively).