Figure 10. Pilot screen results for Spectrum Library.
From the 2320 compound library, 59 compounds (2.5%) were considered hits. 52 of those compounds were confirmed in a single point assay. 6 compounds were found to inhibit either the assay or the Gαi directly, leaving 46 compounds (2.0%). An additional 15 compounds were removed for failing the ALPHA Screen orthogonal assay. And finally, 18 compounds were found to not inhibit RGS4(Δ7) at least 25% in the single point counter screen, leaving 13 compounds (0.6%).