Figure 2. Self-renewal potential of melanoma cells in non-adherent conditions.

SLDA performed after the second reseeding with MSCM revealed that the frequency of cells capable of self renewing was A. 1/216 for the K1735/M4 cell line, B. 1/296 for the K1735/16S cell line and C. 1/74720 for the K1735-16 cell line. The intercept of log (37% negative wells) was used to calculate the sphere-forming frequency (see materials and methods).