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. 2013 Apr 23;8(4):e62124. doi: 10.1371/journal.pone.0062124

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© 2013 Krelin et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Apoptosis was assessed using the TUNEL assay. (A–C) Representative microscopic pictures (magnification X20) of FITC-positive apoptotic tumor cells (green) and counter staining with propidium iodide (red). (D) Percentage of apoptotic cells was quantified by the mean number of FITC-positive cells in 6 microscopic high power fields. Data are expressed as the mean+SD (n = 6 tumors, P<0.001). TUNEL analysis revealed that the number of apoptotic cells in K1735/16 cell line-derived tumors was significantly lower than in K1735/16S and K1735/M4 cells lines-derived tumors.