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. 2013 Apr 23;8(4):e62002. doi: 10.1371/journal.pone.0062002

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© 2013 Imai et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) HPCM1- transplanted mice were treated with 7.5 mg/kg of CDDP for a week, then the generated tumors were analyzed by IHC for CD271. Boxed areas are linked to their respective high-magnification images by horizontal arrows. Scale bar: 100 µm. (B) FACS analyses for CD271 in HPCM1 cells derived from mice with or without CDDP treatment. (C) Expression of ABCC2, ABCB5, and ABCG2 in CD271⁺ and CD271⁻ cells analyzed by real-time RT-PCR. Transcript levels were normalized to those of GAPDH, and the fold increase of each gene expression levels in CD271⁺ cells versus CD271⁻ cells are shown. Values are the mean±SD of triplicate experiments.