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. 2013 Apr 23;8(4):e62138. doi: 10.1371/journal.pone.0062138

Figure 3. Relative luciferase activity of GATA4 wild type and mutant proteins in Hela cells.

Figure 3

(A) Schematic of the human ANF-Luc. The region from the proximal −300 to −130 shows the conserved GATA transcription factor binding sites involved in regulating ANF expression. Two consensus GATA4 binding sites are highlighted in red. (B) Relative luciferase activity of wild type (WT) and CHD specific GATA4 mutant expression constructs that were co-transfected with ANF-Luc in Hela cells. Mean±SD are shown in the histogram. The relative luciferase activity for the A66T mutant was 160±8%, for the T280M mutant was 51±5%, for the A353T mutant was 178±14%, and for the E360G mutant was 78±6%. Student t-test was performed and four mutants caused significant transcriptional activation difference compared with wild-type GATA4 (*, P<0.05). Other mutants did not change the ANF-Luc activation significantly.