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. 2013 Apr 23;8(4):e61857. doi: 10.1371/journal.pone.0061857

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© 2013 Jung et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunofluorescence staining for Stx16 expression in src-shRNA-MDCK and shStx16-MDCK cells. (B) Western blotting of Stx16 expression in src-shRNA-MDCK and shStx16-MDCK cells. (C) Quantitation of band densities from B. Values represent relative protein levels after normalization to an arbitrary value of 100% for src-shRNA expressing cells. The percentage represents the mean (±SD) for n = 3. (D) Transepithelial resistance (TER) measured in monolayers generated from src-shRNA-MDCK and shStx16-MDCK cells at indicated time intervals. (E) Recovery of TER in MDCK monolayers following a Ca²⁺-switch. Stx16 depletion caused a delayed recovery of TER. Graph represents the averages from 3 independent experiments (plus 3 replicates per experiment) and error bars show SEM.