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. 2013 Apr 23;8(4):e61857. doi: 10.1371/journal.pone.0061857

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© 2013 Jung et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunofluorescence-based analysis of E-cadherin localization at MDCK cell-cell junctions during the development and maintenance of a monolayer. (B) Western blotting for E-cadherin following preparation of Triton-soluble (S) and -insoluble (I) fractions from scr-shRNA and shStx16 MDCK confluent monolayer 24 hrs after cell seeding. (C) Densitometric quantitation of E-cadherin bands as in B. Values represent the relative protein levels after normalization to an arbitrary value of 100% for src-shRNA expressing cells. The percentage represents the mean (±SD) for n = 3. (D) Immunofluorescence-based analysis of -catenin localization in scr-shRNA and shStx16 MDCK at various times post cell seeding.