Figure 3. Production of chimeric blastocysts by blastomere aggregation.

(A, D, G) Aggregation of donor (DiI-stained) and host blastomeres between synchronous (A, D) and asynchronous (G) embryonic stages. (B, E, H) Chimeric blastocysts developed from the aggregated blastomeres. (C, F, I) Confocal fluorescence images of the chimeric blastocysts showing DiI fluorescence in ICMs. Single confocal sections of fluorescence were overlaid on the bright field images. Arrow heads, ICM. Scale bars = 50 µm.