Table 1. Summary of localisation patterns displayed by injected RNA.
| Number and proportion of oocytes displaying indicated localisation pattern. | ||||
| Injected RNA and oocyte stage. | Pattern I (Germplasm only) | Pattern II (Germ plasm+late pathway) | Pattern III (latepathway only) | Negative |
| nanos1 FL Stage VI (N = 53) | 268/332 (81%) | 39/332 (12%) | 9/332 (2.7%) | 16/332 (5%) |
| nanos1 3′ UTR Stage VI (N = 30) | 132/243 (54%) | 31/243 (13%) | 50/243 (21%) | 30/243 (12%) |
| nanos1 FL Stage IV (N = 8) | 34/63 (54%) | 17/63 (27%) | 12/63 (19%) | 0/63 (0%) |
| nanos1 3′ UTR Stage IV (N = 7) | 9/56 (16%) | 4/56 (7%) | 32/56 (57%) | 11/56 (20%) |
| Xpat FL alone, Stage VI (N = 9) | 25/49 (51%) | 15/49 (31%) | 0/49 (0%) | 9/49 (18%) |
| Xpat 3′ UTR Stage VI (N = 7) | 30/43 (58%) | 3/43 (4%) | 5/43 (0%) | 5/43 (17%) |
| VegT Stage VI (N = 2) | 7/10 (70%) | 0/10 (0%) | 0/10 (0%) | 3/0 (30%) |
| Vg1 Stage VI (N = 10) | 42/82 (51%) | 11/82 (13%) | 5/82 (6%) | 24/82 (29%) |
| Xvelo1 Stage VI (N = 2) | 4/6 (67%) | 2/6 (33%) | 0/6 (0%) | 0/6 (0%) |
| YFP-Hermes alone, Stage VI (N = 18) | 72/73 (99%) | 1/73 (1%) | 0/73 (0%) | 0/73 (0%) |
| YFP-Hermes alone, Stage IV (N = 3) | 15/15 (100)% | 0/15 (0%) | 0/15 (0%) | 0/15 (0%) |
In each table cell the number of oocytes showing a given localisation with respect to the total number of injected oocytes is shown. N = the number of experiments using oocytes from different females. Cy5 or Cy3-labelled RNAs were routinely injected, with or without RNA encoding YFP-Hermes. The YFP-Hermes alone denotes localisation by the translated protein following injection of the RNA. FL; full length.