Table 2. Summary of localisation patterns displayed by injected mutant RNA.
| Number and proportion of oocytes displaying indicated localisation pattern. | ||||
| Injected RNA. | Pattern I (Germplasm only) | Pattern II (Germ plasm+late pathway) | Pattern III (latepathway only) | Negative |
| nanos1 FL (N = 16) | 63/91 (69%) | 19/91 (21%) | 3/91 (3%) | 6/91 (7%) |
| nanos1-3′Δ1 (N = 5)) | 28/35 (80%) | 0/35 (0%) | 0/35 (0%) | 7/35 (20%) |
| nanos1-3′Δ2 (N = 4) | 26/38 (68%) | 0/38 (0%) | 0/38 (0%) | 12/38 (32%) |
| nanos1-3′UTR FL (N = 15) | 57/90 (63%) | 13/90 (14%) | 5/90 (6%) | 15/90 (17%) |
| nanos1-3′Δ3 (N = 5) | 27/31 (87%) | 0/31 (0%) | 0/31 (0%) | 4/31 (13%) |
| nanos1-3′Δ4 (N = 8) | 46/58 (79.3%) | 3/58 (5%) | 0/58 (0%) | 9/58 (16%) |
| nanos1-3′Δ5 (N = 8) | 0/50 (0%) | 0/50 (0%) | 0/50 (0%) | 50/50 (0%) |
| nanos1-3′Δ6 (N = 14) | 46/102 (45%) | 14/102 (14%) | 3/102 (3%) | 39/102 (38%) |
| mut-nanos1 (N = 8) | 22/38 (58%) | 14/38 (37%) | 0/38 (0%) | 2/38 (5%) |
| nanos1 ORF (N = 5) | 0/24 (0%) | 0/24 (0%) | 0/24 (0%) | 24/24 (100%) |
| nanos1-5′Δ1 (N = 3) | 4/14 (29%) | 4/14 (29%) | 5/14 (36%) | 1/14 (7%) |
| nanos1-5′Δ2 (N = 4) | 9/16 (56%) | 3/16 (19%) | 3/16 (19%) | 1/16 (6%) |
| nanos1-5′Δ3 (N = 3) | 11/14 (79%) | 3/14 (21%) | 0/14 (0%) | 0/14 (0%) |
| nanos1-5′Δ4 (N = 4) | 3/19 (16%) | 0/19 (0%) | 9/19 (47%) | 7/19 (37%) |
In each table cell the number of oocytes displaying a detectable signal with respect to the total number of injected oocytes is shown. The data summarised does not reflect the overall efficiency/strength of localisation by individual mutants, but this was scored as indicated in the key in Figure 7. N = the number of experiments using oocytes from different females. Cy5 or Cy3-labelled RNAs were routinely injected, with or without RNA encoding YFP-Hermes. FL; full length. mut-nanos1 is an RNA with a mutated initiation codon.