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. 2013 Apr 23;8(4):e61790. doi: 10.1371/journal.pone.0061790

Figure 1. Production of hemagglutinin from Influenza strain A/Puerto Rico/8/34 by Schizochytrium sp.

Figure 1

SDS-PAGE analysis of cell-free supernatants under reducing (A and D) and non-reducing (B and C) conditions and corresponding immunoblots with anti-Influenza A virus antibody are shown. Panels “I” are gels stained with Coomassie blue and panels “II” are the corresponding immunoblots. (A) Analysis of cell-free supernatants from Schizochytrium control “C” and experimental “E” strains showing the presence of HA subunits 1 and 2 under reducing conditions HA1 and HA2). (B) Analysis of cell-free supernatants from Schizochytrium control “C” and experimental “E” strains showing the presence of HA under non-reducing conditions. (C) SDS-PAGE analysis (non-reducing) of the Schizochytrium HA fraction used for the mouse challenge study. (D) Analysis of cell-free supernatants from Schizochytrium control “C” and mutant experimental “MutE” strains showing the presence of an uncleaved form of HA (HA0) under reducing conditions.