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. 2013 Apr 23;8(4):e60743. doi: 10.1371/journal.pone.0060743

Figure 4. Characterization of MDCK cell lines stably expressing LMP1.

Figure 4

MDCK cells were transfected with the pSTAR encoding or not LMP1 cDNA After selection with G418, two control clones (V1 and V20) and three LMP1-expressing clones (C9, C15 and C27) were selected and further analyzed with (Dox+) or without (Dox-) doxycycline induction. (A) Expression of LMP1 by Western blot analysis. ERK was used as a loading control. (B) Representative pictures of each cell line. Parental MDCK cells are shown as a reference. (C) Expression of LMP1, E-cadherin and MMP9. ERK was used as a loading control.