Table 2. Sequencing strategies and sequencing efforts of the inversionful chromosome.

Template (bp)	Read (bp)	Num. reads	Seq. depth	Phys. cov.
250 (15)	36	70,014,219	20.22	50.00
250 (15)	75	124,625,311	75.00	50.00
450 (27)	36	69,236,284	20.00	105.00
450 (27)	75	41,541,770	25.00	50.00
450 (27)	150	83,083,540	100.00	50.00
2,500 (150)	36	69,236,284	20.00	674.44
2,500 (150)	75	33,233,416	20.00	313.33
2,500 (150)	150	16,616,708	20.00	146.67
10,000 (599)	36	69,236,284	20.00	2,757.78
10,000 (599)	75	33,233,416	20.00	1,313.33
10,000 (599)	150	16,616,708	20.00	646.67
40,000 (2394)	36	69,236,284	20.00	11,091.11
40,000 (2394)	75	33,233,416	20.00	5,313.33
40,000 (2394)	150	16,616,708	20.00	2,646.67

Sequencing strategies tested, defined by the template and read lengths. Standard deviations of template lengths are shown in brackets. The number of reads simulated from the inversionful chromosome and their corresponding expected sequencing depth and physical coverage are shown.