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. 2013 Apr 23;8(4):e61083. doi: 10.1371/journal.pone.0061083

Table 1. Strains, plasmids, and primers used in this study.

Strain or plasmid Description Reference
SA13011-HeR Heterogeneous-mecA(+) oxacillin susceptible [15]
SA13011-HoR SA13011-HeR+OXA (0.5 µg/ml); SA13011 homogeneous derivative [15]
LMR-21 SA13011-HeR recA-lacZ This study
LMR-22 LMR-21+OXA (0.5 µg/ml); LMR-21 homogeneous derivative This study
LMR-23 SA13011-HeR+pCL15-pbp1T-ANT This study
LMR-24 LMR23+OXA (0.5 µg/ml); LMR-23 homogeneous derivative This study
LMR-25 SA13011-HeR+pCL15-pbp1T-EV (empty-vector) This study
LMR-26 LMR-25+OXA (0.5 µg/ml); LMR-25 homogeneous derivative This study
plasmids
pMC1871 [26]
pRB473 [55]
pCL15 [28]
pMC1871-recA lacZ This study
pRB473-recA lacZ This study
pCL15-pbp1T This study
pCL15-pbp1T-ANT This study
pRB473-recA lacZ This study
Primers
recA-F2 CCTATTGACCCGGGATTAAAAGATG This study
recA-R2 GTTGAAACCCCGGGACCTATAT This study
recA-seq GTTTGTTCGTTTTTGCGTTTTG This study
lacZ-seq CATTAAATGTGAGCGAGTAACAAC This study
pbp1-term-F TAAGTGTGGATCCAGAATTTTAAGGTAG This study
pbp1-term-R GGTGTACGAGCTCTCTTCATATG This study
pbp1F-ans-XbaI CATGGATCTAGAAAGAAGATTTAGAT This study
pbp1R-ans-HindIII CATGGAAAGCTTCCTAAATCTTGACC This study
16S-F TCCGGAATTATTGGGCGTAA This study
16S-R CCACTTTCCTCTTCTGCACTCA This study
recA-F GAGAAATCTTTCGGTAAAGGT This study
recA-R GTGAAGCGCTACTGTTGTCTTACC This study
pbp1-GF AGGTAGCGGTTTTGTGTCC This study
pbp1-GR TATCCTTGTCAGTTTTACTGTC This study
pbp2-GF TATTTAGCCGGTTTACCTCA This study
pbp2-GR TTTTGACGTTCTTCAGGAGT This study
pbp3-GF GTGGACCAACCTCATCTTTA This study
pbp3-GR CGGGAGACCCTTATTATTCT This study
pbp4-GF TGGTGCTAACTGCTTTGTAA This study
pbp4-GF GCTAAAGCTATCGGAATGAA This study