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. 2013 Apr 23;8(4):e61165. doi: 10.1371/journal.pone.0061165

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© 2013 Loureiro et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Immunofluorescence microscopy images of parietal peritoneal sections stained for cytokeratin (green) and FSP-1 (red), with DAPI counterstaining, show accumulation of trans-differentiated mesothelial cells in the submesothelial space at 7, 15 and 30 days of PD mice. Progressive time-dependent increases of MMT and PM thickness is observed during PD fluid exposure. Representative slides are presented. Magnification ×200. (B) Quantification of the submesothelial MMT (cytokeratin/FSP-1 double positive cells per field) at different time points. (C) Quantification of peritoneal thickness ( µm) at different time points. Box Plots represent 25% and 75% percentiles, median, minimum and maximum values. Numbers above boxes depict means ± SE. Symbols show statistical differences between groups. (D) Correlation between both MMT and peritoneal thickness was determined by Spearman regression analysis.