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. 2013 Apr 23;8(4):e61452. doi: 10.1371/journal.pone.0061452

Figure 3. Far-UV CD spectra of Bcl-2 and Bax, and thermal unfolding of the latter, at detergent concentration below CMCBrij-35.

Figure 3

(A) Spectra of 5 µM Bax in the absence (–) and presence (–) of 0.0028% Brij-35, in 25 mM sodium phosphate (pH 7.4), 50 mM NaCl, 1 mM EDTA, and 1 mM DTT, at 20°C. (B) Thermal unfolding of 5 µM Bax in the absence (–) and presence (–) of 0.0028% Brij-35. (C) Far-UV CD spectra of 5 µM Bcl-2 in 0.0028% (–) and 0.05% (–) Brij-35, at 20°C. The composition of the buffer used in experiments (B) and (C) was the same as for (A).