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. 2013 Apr 23;8(4):e61394. doi: 10.1371/journal.pone.0061394

Figure 4. The effect of Ecrg4 expression on mucosal epithelial growth in vitro.

Figure 4

Panel A: Fluorescent images of representative NTHi-infected ME explant outgrowth after transduction with Lenti-ZsGreen or (Panel B) lenti-ZsGreen+Ecrg4 and culture for 10 days. The NTHi-induced hyperplastic growth response is decreased by Ecrg4 gene expression in vitro suggesting that Ecrg4 is a regulatory component of the cellular response to inflammation. Explants of mucosa from MEs were harvested and cultured in vitro for 2 days then transduced. Panel C: Surface area quantification of control (uninfected) explant expansion showing no effect of transduction with ADEcrg4. Panel D: ADEcrg4 transduction dramatically decreases the growth of mucosal explants harvested from NTHi-infected MEs, when compared to those transduced with ADgfp or non-transduced infected explants. In panels C and D, n>6 explants per group per time point, bars represent mean ± SEM with *P<0.05.