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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Clin Immunol. 2013 Mar 15;147(2):79–88. doi: 10.1016/j.clim.2013.02.022

Figure 3. Flow cytometric data showing T cell subsets producing IFN-γ and IL-17 in human peripheral blood.

Figure 3

(A–B) PBMCs were obtained from the peripheral blood of healthy young (A) and elderly (B) individuals. (A) PBMCs were stimulated for 4 hours ex vivo with PMA and ionomycin or PBS (control) in the presence of Golgiplug. Cells were stained with antibodies to CD3, CD4 and CD8. Stained cells were then fixed, permeabilized and stained with antibodies to IL-17 and IFN-γ. The frequency of CD4+ T cells producing IL-17 and/or IFN-γ was measured using flow cytometry.