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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Clin Immunol. 2013 Mar 15;147(2):79–88. doi: 10.1016/j.clim.2013.02.022

Figure 5. Correlation of CD8+ T cell subsets with IgG responses to influenza vaccine in young and elderly people.

Figure 5

PBMCs from healthy young (n = 23) and elderly (n = 26) people were analyzed for naïve (CCR7+CD45RA+), central memory (CM, CCR7+CD45RA−), effector memory (EM, CCR7−CD45RA+/−), IL-7 receptor alpha high and low (IL-7Rαhigh and low) EM CD8+ T cells by flow cytometry before influenza vaccination. Serum levels of IgG specific for influenza virus (A/California/7/2009 (H1N1) and A/Perth/16/2009 (H3N2)) in pre- and post-vaccination sera were determined by ELISA as described in Figure 1. Ratios of pre- and post-vaccination IgG levels were calculated and correlated with the frequency of individual subsets of CD8+ T cells as indicated. Total memory CD8+ T cells include CM and EM CD8+ T cells. P values were determined by Pearson correlation analysis.