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Assessment of cell cycle arrest/apoptosis and DNA damage in adrenals from Sf1-Cre/Dicerlox/lox mice. DAB immunohistochemistry of adrenals Dicer-KO (cre+ KO) embryos and cre− littermate controls (WT) at E14.5, E16.5, and E18.5. A, p21 (Cdkn1a), a cell cycle inhibitor. B, Cleaved-caspase 3, an apoptotic marker. C, Phospho-γ-H2A.X, an indicator of DNA damage repair. Tissues were counterstained with hematoxylin or eosin. Images are taken at ×100 magnification.
