Skip to main content
. 2013 Mar 21;27(5):754–768. doi: 10.1210/me.2012-1331

Figure 5.

Figure 5.

Quantitative real-time PCR to confirm Nr6a1 expression in adrenals from Sf1-Cre/Dicerlox/lox and Sf1-Cre–only tissues. Total RNA was isolated from adrenals and reverse transcribed and quantitative PCR performed as described in Materials and Methods. A, Comparison of Nr6a1 expression in adrenals from adult mice expressing only the Sf1-Cre transgene (Cre), relative to WT animals. B and C, Expression of the three Nr6a1 transcript isoforms in 4 individual samples of E15.5 and E16.5 Sf1-Cre/Dicerlox/lox adrenals relative to their corresponding WT control samples. Statistical significance was determined using unpaired t tests. *, P < .05; **, P ≤ .01; ***, P ≤ .001.