Table 1.

Phenotypic analysis showing reduced abundance of PPTC staining for two NAC-domain and two MYB-related genes identified by bioinformatics as candidate transcriptional regulators of wall ingrowth deposition in PPTCs of Arabidopsis leaf veins.

Gene number	Mutant allele	% vein length showing staining for PPTCs§
WT (Col-0)		45.3 ± 3.6
NAC-domain
At3g04420	FLAG_009F02	18.2 ± 3.8**
At1g33060	SALK_085596	27.5 ± 2.9*
At1g33060	SALK_024241	31.5 ± 4.7*
MYB-related
At1g25550	SALK_144656	16.3 ± 2.4**
At1g49560	SALK_085182	20.5 ± 2.9*
At1g49560	SALK_095775	15.6 ± 2.7**
GIGANTEA
At1g22770	gi-2	3.3 ±1.0**,†

^§ This value was measured from mature, Aniline Blue-stained leaves according to our previously published method (Edwards et al., 2010). Data is presented as mean ± SE from two leaves from each of three plants per line.

^*P < 0.01,

^**P < 0.001.

^†Data for gi-2 taken from Edwards et al. (2010).