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C18 RP-HPLC and ESI-MS analysis of purified recombinant pro-cathelicidin, LL-37 and hCLD. Proteins were analyzed on a Waters XBridge™ BEH130 C18 column (4.6 × 150 mm, 3.5μm) using a linear gradient of 5 – 65% acetonitrile at a flow rate of 1 mL/min over 30 min. The experimental values of the molecular masses of the desired products are shown together with expected values calculated based on the average isotope compositions (in parentheses).
