Table 3.

LRAP layer protein coverage and hydration obtained by assuming protein SLD values. The protein SLDs were calculated assuming 0% or 100% deuteration of LRAP by exchange of labile protons with D₂O solvent (Table S2).

Model	%D exchange	Layer	Protein SLD × 106 (Å−2)	LRAP coverage (v%)	Hydration (v%)	gH2O/g protein1
One layer protein	0		2.16	70.2±0.04	29.8±0.4	0.32
	100		3.13	74.1±1.2	20.7±0.5	0.21
Two layer monomer-inner high protein SLD2	0	Inner	2.31	65.5±1.2	32.0±0.6	0.37
		Outer	1.81	80.5±5.0	18.7±1.4	0.17
	100	Inner	3.82	63.5±1.7	25.0±1.1	0.29
		Outer	2.55	73.6±1.6	13.1±0.9	0.13
Two layer monomer-inner low protein SLD3	0	Inner	1.81	30.24±22.5	60±18	1.49
		Outer	2.31	66.3±3.4	29.5±3.1	0.33
	100	Inner	2.55	74.65±1.31	25.2±0.94	0.26
		Outer	3.82	89.22±1.63	10.6±1.2	0.09

¹Degree of hydration calculated in terms of grams of water (H₂O) per grams of protein.

²Protein SLDs were calculated assuming the inner layer was the C-terminus (42–59) and inner N-terminus (8–24) and the outer layer was the outer N-terminus (1–7) and middle hydrophobic region (25–41) as described in the text and supplementary section.

³Protein SLDs were calculated assuming the protein was flipped - inner layer was the N-terminus (1–7) and middle hydrophobic region (25–41) and the outer layer was the C-terminus (42–59) and inner N-terminus (8–24).