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. 1988 Jun;8(6):2361–2366. doi: 10.1128/mcb.8.6.2361

Group II intron domain 5 facilitates a trans-splicing reaction.

K A Jarrell 1, R C Dietrich 1, P S Perlman 1
PMCID: PMC363434  PMID: 3405208

Abstract

A self-splicing group II intron of yeast mitochondrial DNA (aI5g) was divided within intron domain 4 to yield two RNAs that trans-spliced in vitro with associated trans-branching of excised intron fragments. Reformation of the domain 4 secondary structure was not necessary for the trans reaction, since domain 4 sequences were shown to be dispensable. Instead, the trans reaction depended on a previously unpredicted interaction between intron domain 5, the most highly conserved region of group II introns, and another region of the RNA. Domain 5 was shown to be essential for cleavage at the 5' splice site. It stimulated that cleavage when supplied as a trans-acting RNA containing only 42 nucleotides of intron sequence. The relevance of our findings to in vivo trans-splicing mechanisms is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Fox T. D., Leaver C. J. The Zea mays mitochondrial gene coding cytochrome oxidase subunit II has an intervening sequence and does not contain TGA codons. Cell. 1981 Nov;26(3 Pt 1):315–323. doi: 10.1016/0092-8674(81)90200-2. [DOI] [PubMed] [Google Scholar]
  2. Fromm H., Edelman M., Koller B., Goloubinoff P., Galun E. The enigma of the gene coding for ribosomal protein S12 in the chloroplasts of Nicotiana. Nucleic Acids Res. 1986 Jan 24;14(2):883–898. doi: 10.1093/nar/14.2.883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Inoue T., Sullivan F. X., Cech T. R. Intermolecular exon ligation of the rRNA precursor of Tetrahymena: oligonucleotides can function as 5' exons. Cell. 1985 Dec;43(2 Pt 1):431–437. doi: 10.1016/0092-8674(85)90173-4. [DOI] [PubMed] [Google Scholar]
  4. Jacquier A., Michel F. Multiple exon-binding sites in class II self-splicing introns. Cell. 1987 Jul 3;50(1):17–29. doi: 10.1016/0092-8674(87)90658-1. [DOI] [PubMed] [Google Scholar]
  5. Jacquier A., Rosbash M. Efficient trans-splicing of a yeast mitochondrial RNA group II intron implicates a strong 5' exon-intron interaction. Science. 1986 Nov 28;234(4780):1099–1104. doi: 10.1126/science.2430332. [DOI] [PubMed] [Google Scholar]
  6. Jarrell K. A., Peebles C. L., Dietrich R. C., Romiti S. L., Perlman P. S. Group II intron self-splicing. Alternative reaction conditions yield novel products. J Biol Chem. 1988 Mar 5;263(7):3432–3439. [PubMed] [Google Scholar]
  7. Kao T., Moon E., Wu R. Cytochrome oxidase subunit II gene of rice has an insertion sequence within the intron. Nucleic Acids Res. 1984 Oct 11;12(19):7305–7315. doi: 10.1093/nar/12.19.7305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Koller B., Fromm H., Galun E., Edelman M. Evidence for in vivo trans splicing of pre-mRNAs in tobacco chloroplasts. Cell. 1987 Jan 16;48(1):111–119. doi: 10.1016/0092-8674(87)90361-8. [DOI] [PubMed] [Google Scholar]
  9. Konarska M. M., Padgett R. A., Sharp P. A. Trans splicing of mRNA precursors in vitro. Cell. 1985 Aug;42(1):165–171. doi: 10.1016/s0092-8674(85)80112-4. [DOI] [PubMed] [Google Scholar]
  10. Krause M., Hirsh D. A trans-spliced leader sequence on actin mRNA in C. elegans. Cell. 1987 Jun 19;49(6):753–761. doi: 10.1016/0092-8674(87)90613-1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kück U., Choquet Y., Schneider M., Dron M., Bennoun P. Structural and transcription analysis of two homologous genes for the P700 chlorophyll a-apoproteins in Chlamydomonas reinhardii: evidence for in vivo trans-splicing. EMBO J. 1987 Aug;6(8):2185–2195. doi: 10.1002/j.1460-2075.1987.tb02489.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A. 1985 Oct;82(20):6955–6959. doi: 10.1073/pnas.82.20.6955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Michel F., Dujon B. Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast- and nuclear-encoded members. EMBO J. 1983;2(1):33–38. doi: 10.1002/j.1460-2075.1983.tb01376.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Murphy W. J., Watkins K. P., Agabian N. Identification of a novel Y branch structure as an intermediate in trypanosome mRNA processing: evidence for trans splicing. Cell. 1986 Nov 21;47(4):517–525. doi: 10.1016/0092-8674(86)90616-1. [DOI] [PubMed] [Google Scholar]
  15. Peebles C. L., Perlman P. S., Mecklenburg K. L., Petrillo M. L., Tabor J. H., Jarrell K. A., Cheng H. L. A self-splicing RNA excises an intron lariat. Cell. 1986 Jan 31;44(2):213–223. doi: 10.1016/0092-8674(86)90755-5. [DOI] [PubMed] [Google Scholar]
  16. Perlman P. S., Jarrell K. A., Dietrich R. C., Peebles C. L., Romiti S. L., Benatan E. J. Mitochondrial gene expression in yeast: further studies of a self-splicing group II intron. Basic Life Sci. 1986;40:39–55. doi: 10.1007/978-1-4684-5251-8_4. [DOI] [PubMed] [Google Scholar]
  17. Schmelzer C., Schmidt C., May K., Schweyen R. J. Determination of functional domains in intron bI1 of yeast mitochondrial RNA by studies of mitochondrial mutations and a nuclear suppressor. EMBO J. 1983;2(11):2047–2052. doi: 10.1002/j.1460-2075.1983.tb01698.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schmelzer C., Schweyen R. J. Self-splicing of group II introns in vitro: mapping of the branch point and mutational inhibition of lariat formation. Cell. 1986 Aug 15;46(4):557–565. doi: 10.1016/0092-8674(86)90881-0. [DOI] [PubMed] [Google Scholar]
  19. Sharp P. A. On the origin of RNA splicing and introns. Cell. 1985 Sep;42(2):397–400. doi: 10.1016/0092-8674(85)90092-3. [DOI] [PubMed] [Google Scholar]
  20. Sharp P. A. Trans splicing: variation on a familiar theme? Cell. 1987 Jul 17;50(2):147–148. doi: 10.1016/0092-8674(87)90207-8. [DOI] [PubMed] [Google Scholar]
  21. Solnick D. Trans splicing of mRNA precursors. Cell. 1985 Aug;42(1):157–164. doi: 10.1016/s0092-8674(85)80111-2. [DOI] [PubMed] [Google Scholar]
  22. Sutton R. E., Boothroyd J. C. Evidence for trans splicing in trypanosomes. Cell. 1986 Nov 21;47(4):527–535. doi: 10.1016/0092-8674(86)90617-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. van der Veen R., Arnberg A. C., van der Horst G., Bonen L., Tabak H. F., Grivell L. A. Excised group II introns in yeast mitochondria are lariats and can be formed by self-splicing in vitro. Cell. 1986 Jan 31;44(2):225–234. doi: 10.1016/0092-8674(86)90756-7. [DOI] [PubMed] [Google Scholar]

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