Figure 4.

In vitro assays suggest that Lu AF20513 induces the production of functionally potent anti-Aβ antibody. A, Purified anti-Aβ antibody binds to monomeric, oligomeric, and fibrillar forms of Aβ₄₂ with high affinity. The black lines represent individual data points and the orange lines represent fitted curves B. Binding kinetics (K_D is the affinity constant, k_a the association rate, and k_d the dissociation rate) of purified anti-Aβ and 6E10 antibodies to different forms of Aβ₄₂. C, Anti-Aβ antibody, but not irrelevant mouse IgG, binds to cortical plaques in AD brain. Original magnification is 10×. Scale bar, 100 μm. D, Purified anti-Aβ antibody inhibits neurotoxicity mediated by 2 μm Aβ₄₂ (fibrillar or oligomeric forms). Data were collected from four replicates and are expressed as a percentage of control ± SD. ****p ≤ 0.0001.