. Author manuscript; available in PMC: 2014 May 1.

Published in final edited form as: J Hepatol. 2013 Jan 20;58(5):993–999. doi: 10.1016/j.jhep.2013.01.011

Table 1.

Fold of change in the expression level of genes involved in lipogenesis and fatty acid oxidation

	CBZ^#	Rapamycin^#	29% ED^$	HFD^*
Srebp-1c	1.18±0.13	1.45±0.46	1.33±0.33	2.77±0.29^a
Fasn	0.97±0.17	1.53±0.61	3.41±1.72	1.88±0.11^a
Acc1	0.84±0.11	0.94±0.21	0.48±0.06^a	0.82±0.08
Acox1	0.80±0.34	1.80±0.80	1.27±0.13	1.31±0.08^b
Cpt1a	1.18±0.25	3.67±1.58	0.58±0.07^a	1.21±0.06^b

Hepatic RNA were isolated and subjected to quantitative RT-PCR for the genes indicated. The expression levels were normalized with cyclophilin A and compared to untreated controls, which is set at 1.0. Srebp-1c, sterol regulatory element binding protein-1c, Fasn: fatty acid synthase, Acc1: acetyl-CoA carboxylase 1, Acox1: acyl-CoA oxidase 1, Cpt1a: carnitine-palmitoyl transferase, 1a. ND: not determined. Values shown are mean±SEM.

Letter a indicates p<0.01, letter b indicates p<0.05 (t test).

Mice were given CBZ (25 mg/kg) or rapamycin (2 mg/kg) by i.p. and sacrificed 16 hours later. Expression levels are compared to control mice without treatment (n=3–6).

Mice were given 29% ethanol liquid diet for 4 weeks. Expression levels were compared to mice receiving control liquid diet (n=3).

Mice were given HFD for 20 weeks. Expression levels were compared to mice receiving regular chow diet (n=4–6).