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. 2013 Mar 21;14(3):6436–6453. doi: 10.3390/ijms14036436

Figure 1.

Figure 1

Figure 1

Effects of TthMutS on standard polymerase chain reaction (PCR) amplification of an 80-bp template. (A) Schematic representation of the primers and templates used in (B,C). Perfectly matched, GT-mismatched or unpaired T-containing primers were used; (B) Perfectly matched (left), GT-mismatched (middle) or unpaired T-containing (right) primers were used to amplify the perfectly matched template; (C) The relative amounts of the products from perfectly matched (blue), GT-mismatched (red) or unpaired T-containing (purple) primers were plotted against the TthMutS concentration for reactions using three polymerases: LA Taq (left), KOD polymerase (middle) and A. aeolicus DnaE (right). The amounts of the products were normalized by those at 0 μM TthMutS.