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. 2013 Mar 21;14(3):6436–6453. doi: 10.3390/ijms14036436

Figure 5.

Figure 5

Effect of the number of mismatches in an 80-bp template on amplification efficiency in the presence of TthMutS. (A) Templates containing zero, one, two or three unpaired T mismatches were used. PCR was performed using LA Taq Hot-Start version with perfectly matched primers in the absence or presence of 0.35 μM TthMutS; (B) Quantification of the relative amounts of amplified fragments. Gray and blue columns indicate amplification in the absence or presence of TthMutS, respectively. The amounts of the products were normalized by that from perfectly-matched template at 0 μM TthMutS; (C) Model for the mechanism by which TthMutS suppresses amplification from mismatched templates.