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(a) Images of wound closure assay of TOV21G cell line stably transfected with PAX2 specific shRNAs during a 24 h period. PLKO and non-target shRNA were the negative controls, and shRNAs 15839, 15840 and 15841 were PAX2-targeted shRNAs; (b) the images were analyzed by the TScratch program [26] to estimate the percentage of wound closure. PAX2 stable knockdown TOV21G cells had slower rate of wound closure.
