Performance of OsFKBP16-3-overexpressing transgenic rice under environmental stress. (A,C,E) Comparison of rice seedlings under NaCl, mannitol and methyl viologen (MV) stress. Transgenic plants were selected for three days on MS medium containing hygromycin (50 mg/L) and grown on fresh MS medium containing 0, 150 or 200 mM NaCl for 10 days (A); 200 mM mannitol for seven days (C) or 2.5 or 5 μM MV for seven days (E); (B,D,F) Fresh weights of transgenic and wild-type seedlings under environmental stress. WT: wild-type rice (Dongjin ecotype); RT: OsFKBP16-3-overexpressing transgenic rice. Error bars represent SD; asterisks indicate significant differences from the vector control using t-test p-value. (*t-test, with p < 0.1, **t-test, with p < 0.05).