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. 2013 Mar 13;14(3):5899–5919. doi: 10.3390/ijms14035899

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

PMC Copyright notice

Expression and redox state of recombinant his-tagged mature form OsFKBP16-3 protein (mOsFKBP16-3) in E. coli. (A) Recombinant mOsFKBP16-3 protein expression was induced with isopropyl β-D-1-thiogalactopyranoside (IPTG) in E. coli. Samples were separated by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under non-reducing conditions and stained with Coomassie blue. The reduction of his-tagged mOsFKBP16-3 protein was accomplished by treatment with 100 mM DTT; (B) Immunoblot of expressed OsFKBP16-3 protein probed with the monoclonal his antibody; (C) Two cysteine-to-serine mutation was carried out in mOsFKBP16-3 proteins. The mutant protein was expressed and treated with DTT as wild-type mOsFKBP16-3. WT: wild-type mOsFKBP 16-3 protein; C128,131S: cysteine to serine mutant protein of mOsFKBP16-3.