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. 2013 Feb 26;14(3):4613–4628. doi: 10.3390/ijms14034613

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Effect of the coupling time on the amount of immobilized BlGGT (closed circles) and activity recovery (open circles). In this experiment, the initial enzyme concentration was 0.92 mg/mL.