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Lineweaver-Burk plot of the GSTs activity in crude enzyme extract. (A) Specific activity of GSTs with and without cantharidin. IC50 value of cantharidin for the GSTs using glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) secondary substrate; (B) IC50 value was obtained using a plot of percent activities vs. varying concentrations of cantharidin. S-Hexylglutathione (GTX) was used as positive control.
