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. 2013 Feb 25;14(3):4461–4475. doi: 10.3390/ijms14034461

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© 2013 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Effects of nolinospiroside F on the replicative lifespan of sod1 (A) and sod2 (B) mutant yeast strains with a K6001 background. The assay is described in Figure 2. The daughter cells of 40 microcolonies of each experiment were counted. The assay was repeated at least thrice. The average lifespan of untreated K6001 was 6.35 generations; RES at 10 μM, 8.83***; nolinospiroside F at 3 μM, 8.75***; sod1 mutant with a K6001 background was 3.50; nolinospiroside F at 3 μM, 2.83; sod2 mutant with a K6001 background was 5.60; nolinospiroside F at 3 μM, 5.80. Δsod1 and Δsod2 represent the sod1 and sod2 mutants yeast strain with a K6001 background, respectively. (*** p < 0.001).