Figure 6. Raw traces of wild-type (WT; A and B) and endothelial nitric oxide synthase (eNOS)^−/− (C and D) lymphatic vessel contractile activity during ACh dose–response tests, conducted in the absence and presence of l-NAME.

Input and output pressures, overlaid in the top trace, were fixed at 3 cmH₂O for the entire protocol. The diameter traces are plotted over time, on the bottom. Vertical lines in the diameter traces mark the application of each dose (labelled) of ACh to the bath. Traces in A and B are from a single WT vessel, and traces in C and D are from a single eNOS^−/− vessel.