Figure 3. NBCn1 is expressed in the basolateral membrane of colonic crypt cells but appears not to be significantly involved in pHi recovery from intracellular acid loads.
A, NBCn1 staining in colonic mucosa is markedly weaker than in the duodenum, and is crypt predominant. Scale bars represent 100 μm.B, representative pHi trace of cells at the base of the colonic crypts from mid-distal colon during an ammonium prepulse and recovery phase in the presence of inhibitors for the colonic NHEs (Bachmann et al. 2004). C, the low non-NHE-mediated pHi recovery rate from intracellular acidification was not different in NBCn1 KO and WT crypts. n= 5.