Table 1.

Tally of orexin-A-ir neurons in WT and orexin-ataxin-3 mice

	Group	Average no. of orexin-ir neurons
1	No rAAV (n = 7)	0.76 (0.47)*
2	rAAV-GFP (n = 10)	0.50 (0.21)*
3	rAAV-MCH-orexin (30% of MCH neurons) (n = 9)	31.08 (5.53)*
4	Dilute rAAV-orexin in LH (n = 6)	55.56 (8.3)**
5	rAAV-MCH-orexin (65% of MCH neurons) (n = 7)	94.76 (11.55)***
6	rAAV-orexin in striatum (n = 9)	115.45 (17.57)****
7	rAAV-orexin in ZI (n = 8)	138.31 (12.24)****
8	rAAV-orexin in LH (n = 11)	179.4 (25.75)*****
9	WT (n = 6)	121.81 (8.71)

All orexin-A-ir neurons were counted in both hemispheres. An average count per section was determined for each mouse. The table summarizes the group average (±SEM).

One-way ANOVA (F_(8,72) = 20.07; p < 0.001) determined significant difference between groups.

*Versus groups 5–9;

**versus groups 6–9 (p < 0.04);

***versus groups 1, 2, 3, 8 (p < 0.03);

****versus 1, 2, 3, 4, 8 (p < 0.03);

*****versus all groups (p < 0.05).