Table 2.

Relative density of orexin-A-immunoreactive fibers in WT and orexin-ataxin-3 mice given the orexin gene transfer

Region	WT (n = 5)	rAAV-MCH-orexin in LH (n = 7)	rAAV-orexin in striatum (n = 9)	Dilute rAAV-orexin LH (n = 6)	rAAV-orexin LH (n = 11)	rAAV-orexin ZI (n = 8)
Caudate	0	+	++++	0	0	0
Lateral septum	+	+	0	++	++	++
Medial septum	++	++	+	+	++	++
SI	++	++	++	+	+++	++
VDB	++	++	++	+	+++	++
HDB	++	++	++	+	+++	++
VLPO/mnPO	+	++	++	++	+++	++
CEA	+	++	0	+	++	++
Anterior dorsal thalamus	+	+	0	++	+++	++
Ventral medial thalamus	+	+	0	++	++++	++++
Ventral lateral thalamus	+	+	0	++	++	+++
PVA	++	+	+	+	++	++
ZI	+	+	0	+	+	++++
LH	++++	++++	+	++++	++++	++
DMH	++	++++	0	++++	++++	++
VMH	+	++	0	+	+++	++
Arcuate nucleus	++	++	0	++	+++	++
TMN	+++	++	++	++++	++++	+++
VTA	+	+	+	+	+	++
SN reticulata	0	+	+++	0	+	+
SN compacta	+	+	+	0	+	++++
VLPAG	++	++	+	++	++	++
DRN	+++	++	+	++	++	++
LDT	++	++	0	+	+	++
SLD	++	+	0	+	+	++
Gudden's nucleus	0	0	0	+	++	0
LC	+++	++	+	++	++	++

The rAAV-MCH-orexin group represents 65% of MCH neurons that were also orexin-A-ir (the other MCH group was not evaluated). The no-rAAV and rAAV-GFP groups are not represented here because orexin-ir fibers were not evident in these groups. The following four-point scale was used to evaluate density: orexin-A-ir somata along with fibers, ++++; heavy orexin-a-ir fibers, +++; moderate orexin-A-ir fibers, ++; few orexin-A-ir fibers, +; no orexin-A-ir fibers, 0.