Table 4.
Length of bouts of the five vigilance states during the 12 h day and night periods
| Group | Length of bouts (s) |
|||||
|---|---|---|---|---|---|---|
| Cataplexy | REM sleep | Wake | Non-REM sleep | Sleep attacks | ||
| During the night | ||||||
| 1 | WT (n = 5) | NA | 59.02 (3.21) | 449.53 (75.95)‡ | 129.07 (9.83) | NA |
| 2 | No rAAV (n = 7) | 79.29 (7.26) | 71.31 (5.70) | 264.27 (24.10) | 118.95 (9.96) | 38.10 (1.86) |
| 3 | rAAV-GFP (n = 10) | 73.52 (4.8) | 64.12 (3.79) | 241.29 (27.56) | 119.61 (6.19) | 35.75 (4.1) |
| 4 | rAAV-orexin in striatum (n = 9) | 99.09 (4.13) | 53.09 (2.04)† | 233.43 (20.10) | 130.17 (6.70) | 37.01 (3.18) |
| 5 | rAAV-MCH-orexin (30% of MCH neurons) (n = 9) | 81.49 (4.80) | 51.08 (4.05)†† | 261.75 (28.25) | 116.34 (7.46) | 35.65 (2.04) |
| 6 | rAAV-MCH-orexin (65% of MCH neurons) (n = 7) | 58.33 (4.88)* | 41.46 (23.55)††† | 216.77 (27.1) | 105.38 (7.86) | 33.16 (2.32) |
| 7 | Dilute rAAV-orexin in LH (n = 6) | 87.64 (7.69) | 57.83 (2.2) | 259.86 (24.70) | 104.32 (8.32) | 30.77 (2.84) |
| 8 | rAAV-orexin in LH (n = 11) | 53.62 (6.64)** | 50.79 (1.33)†† | 261.51 (22.26) | 123.25 (4.94) | 34.55 (2.51) |
| 9 | rAAV-orexin in ZI (n = 8) | 44.69 (8.02)** | 54.77 (2.97)† | 234.02 (20.04) | 118.62 (4.41) | 37.02 (2.69) |
| During the day | ||||||
| 1 | WT (n = 5) | NA | 63.14 (3.70)¶ | 105.54 (6.54) | 157.67 (8.45) | NA |
| 2 | No rAAV (n = 7) | 49.29 (13.87) | 56.43 (2.57)¶ | 96.22 (4.24) | 130.40 (5.17) | 31.5 (2.47) |
| 3 | rAAV-GFP (n = 10) | 82.24 (13.92) | 48.15 (3.81) | 113.10 (14.29) | 128.97 (9.2) | 23.15 (2.2) |
| 4 | rAAV-orexin in striatum (n = 9) | 86.68 (5.35) | 46.37 (1.23) | 96.51 (4.09) | 147.67 (4.8) | 34.84 (1.64) |
| 5 | rAAV-MCH-orexin (30% of MCH neurons) (n = 9) | 61.32 (8.24) | 40.75 (1.57) | 114.48 (8.79) | 146.93 (9.6) | 28.67 (4.02) |
| 6 | rAAV-MCH-orexin (65% of MCH neurons) (n = 7) | 50.82 (2.33) | 37.84 (2.56) | 133.41 (10.85) | 158.89 (9.33) | 30.96 (6.34) |
| 7 | Dilute rAAV-orexin in LH (n = 6) | 50.50 (18.79) | 48.65 (1.99) | 107.19 (6.30) | 127.69 (4.44) | 25.73 (4.85) |
| 8 | rAAV-orexin in LH (n = 11) | 28.61 (7.66)§ | 47.36 (1.43) | 118.17 (13.81) | 140.22 (4.98) | 30.22 (1.97) |
| 9 | rAAV-orexin in ZI (n = 8) | 22.50 (9.80)§ | 43.22 (1.47) | 106.77 (9.64) | 133.16 (3.23) | 24.56 (4.22) |
Values represent length (in seconds) ± SEM. Each vigilance state was identified in 12 s epochs, and average length of the bouts during the 12 h day and night periods was determined for each group. Cataplexy and sleep attacks did not occur in WT mice and is not applicable (NA).
*Versus 4, 7;
**versus 2–5, 7;
†versus 2;
††versus 2, 3;
†††versus 1, 2, 3, 7;
‡versus 2–9 (p < 0.003);
§versus 3, 4;
¶versus 3–9 (p < 0.002).