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Structures of complexes used for bacteria labeling studies. The ZnII-binding domains of the complexes are shown in blue, the GdIII chelate is shown in red. After the addition of ZnII, Zn-1 and Zn-2 have charges of 4+ and 2+, respectively, while Zn-3 is charge neutral.[16] The positive charge of Zn-1 and Zn-2 is expected to enhance the complex's affinity for anionic bacterial cell membranes. Zn-3 was used as a control for low-affinity binding.
