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Top: aromatic region of 1H NMR spectrum of Eu-1 with increasing amounts of ZnII. Bottom: Chemical shift of peaks c, e, f, and d (see Figure 3) as a function of ZnII/Eu-1 ratio. It is clear that the peaks of the ZnII binding domain shift only until a ZnII/Eu-1 ratio of 2 is obtained, confirming that each Eu-1 binds two ZnII ions.
