FIG 2 .

Contribution of individual SPI-2 T3SS effectors to S. Typhimurium intracellular replication. Fluorescence Dilution analysis of the replication of S. Typhimurium strains carrying deletions in genes of effectors of the SPI-2 T3SS was done in bone marrow-derived macrophages over 17 h. The color code used to group SPI-2 T3SS effectors according to their function is as follows: SCV membrane dynamics, green; SCV localization, yellow; cell migration, red; ubiquitin pathways, purple; unknown function, dark gray; actin cytoskeleton, brown; host immune signaling, pink; SPI-1 translocated effectors, turquoise. Data are expressed as the means ± SEM of the results of 3 to 30 independent experiments (N) and were normalized to wild-type values. Statistical analysis was performed with one-way analysis of variance (ANOVA) and a post hoc Dunnett test (*, P < 0.05; **, P < 0.01) relative to the wild-type strain results.